Journal: Cells

Article Title: Allogeneic Adipose-Derived Mesenchymal Stem Cell Transplantation Alleviates Atherosclerotic Plaque by Inhibiting Ox-LDL Uptake, Inflammatory Reaction and Endothelial Damage in Rabbits

doi: 10.3390/cells12151936

Figure Lengend Snippet: Detection of inflammatory cells and cytokines in aorta. Staining and analysis of macrophage marker molecules CD68, iNOS and Arginase-1 ( A ), inflammatory factors TNF-α and IL-6, and anti-inflammatory factors IL-10 and TGF-β ( B ): the levels of CD68, iNOS, IL-6 and TNF-α were lower, and the levels of IL-10 and TGF-β were higher in the T group compared with those in the model group, respectively (the areas of atherosclerotic plaque were circled with dashed lines, and indicated by green arrows) (n = 5–6 in each group). ** p < 0.01, bar = 100 µm.

Article Snippet: The cells were fixed in a 4% paraformaldehyde (E672002, Sangon Biotech), washed with PBS, blocked with serum for 30 min, incubated overnight with anti-arginase 1 (bs-23837R, Bioss) and iNOS (bs-0162R, Bioss) antibodies at 4 °C.

Techniques: Staining, Marker

Journal: Cells

Article Title: Allogeneic Adipose-Derived Mesenchymal Stem Cell Transplantation Alleviates Atherosclerotic Plaque by Inhibiting Ox-LDL Uptake, Inflammatory Reaction and Endothelial Damage in Rabbits

doi: 10.3390/cells12151936

Figure Lengend Snippet: ADSC and macrophage polarization assay in vitro. ( A – C ) Immunofluorescence detection of M1, M2 macrophage marker proteins iNOS and arginase-1. Compared with the model group, iNOS level was lower, and arginase-1 expression was higher in ADSC treatment group. Original magnification is 200. ( D ) ELISA results suggested that level of cytokine TNF-α secreted by M1 macrophages decreased in ADSCs group. ( E ) Western blot detection showed the expression of STAT6 signal molecules increased significantly in ADSCs group. * p < 0.05, ** p < 0.01.

Article Snippet: The cells were fixed in a 4% paraformaldehyde (E672002, Sangon Biotech), washed with PBS, blocked with serum for 30 min, incubated overnight with anti-arginase 1 (bs-23837R, Bioss) and iNOS (bs-0162R, Bioss) antibodies at 4 °C.

Techniques: In Vitro, Immunofluorescence, Marker, Expressing, Enzyme-linked Immunosorbent Assay, Western Blot

Journal: Cells

Article Title: Allogeneic Adipose-Derived Mesenchymal Stem Cell Transplantation Alleviates Atherosclerotic Plaque by Inhibiting Ox-LDL Uptake, Inflammatory Reaction and Endothelial Damage in Rabbits

doi: 10.3390/cells12151936

Figure Lengend Snippet: ADSC-derived exosomes and macrophage polarization assay in vitro. ( A ) TEM analysis presented that ADSC-derived exosomes were round membrane-bound vesicles and the diameter is about 30 mm to 150 nm as indicated by the red arrow. Scale bar is 200 nm. ( B ) Exosomes from rabbit ADSCs were identified by markers CD9, HSP70, TSG101 and Calnexin by Western blot. ( C , F ) Immunofluorescence detection of M1, M2 macrophage marker proteins iNOS and arginase-1. Compared with the model group, iNOS level was lower, and arginase-1 expression was higher in ADSC exosome treatment group. Original magnification is 200. ( D , E ) Western blot detection showed the expression of STAT6 signal molecules increased significantly in ADSC-derived exosome group. ( G ) ELISA results suggested that concentration of cytokine TNF-α decreased and IL-10 increased in ADSC exosomes group. * p < 0.05.

Article Snippet: The cells were fixed in a 4% paraformaldehyde (E672002, Sangon Biotech), washed with PBS, blocked with serum for 30 min, incubated overnight with anti-arginase 1 (bs-23837R, Bioss) and iNOS (bs-0162R, Bioss) antibodies at 4 °C.

Techniques: Derivative Assay, In Vitro, Western Blot, Immunofluorescence, Marker, Expressing, Enzyme-linked Immunosorbent Assay, Concentration Assay